Having established in this system that treatment with WORT impairs autophagy, it was then necessary to determine whether caspase 1 mediated pyroptosis30 or caspase 3 mediated apoptosis was evoked and contributed to RPE death. Immuno staining revealed only caspase 3, but not caspase 1, activation in cells pretreated with WORT (Fig. 1E) or CQ (Supplementary Fig.
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