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The mean fluorescence of uncloned, and unselected, EGFP expressing cells was determined 4 days after transduction. Strikingly, the mean fluorescence showed a highly significant increase when more than 40% of cells were EGFP+, as obtained with a MOI >1 (Figure 2a The increase in expression was almost linear when the MOI exceeded 3, very similar to the data obtained by others using lacZ vectors.12 Thus, a high MOI in the range of 10 produced at least a log difference in the mean fluorescence. In contrast, the expression levels were almost constant when the MOI was adjusted between 0.03 and 0.5, resulting in 3 of cells being EGFP+.

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